How Do Gene Expression Patterns Change in Response to Osmotic Stresses in Kuruma Shrimp (Marsupenaeus japonicus)?

Euryhaline crustaceans cope with external salinity changes by mechanisms of osmoregulation. In the current study, we first cloned and confirmed ORF sequences ion-transportation-related genes Na+/K+-ATPase ? subunit (NKA?), cytoplasmic carbonic anhydrase (CAc), V-type H+-ATPase G (VHA-G), water channels aquaporins (AQP3, AQP4, AQP11) from kuruma shrimp (Marsupenaeus japonicus). Further tissue expression patterns showed a higher MjAQP4, MjCAc, MjNKA?, MjVHA-G in gills, as well MjAQP3 MjAQP11 intestine muscle, respectively. Then, qPCR analysis was used to assess mRNA levels those osmoregulatory both post-larvae adult when they were exposed acute stress or acclimation. The results revealed significantly decreased MjAQP3, MjAQP11, MjAQP4 directly subjected 10‰ 50‰ salinity. Moreover, similar also observed during accommodation As shrimp, gills after exposure salinity, whereas only MjNKA? up-regulated at 40‰ contrast, Finally, acclimation elevated, while MjCAc gills. addition, 55‰ findings study suggest that examined are critical for adaptation aquatic changing environmental Our lays foundation further research on osmoregulation M. japonicus.